Composite

Part:BBa_K3352008:Design

Designed by: Tsuyoshi Misaki   Group: iGEM20_TAS_Taipei   (2020-10-18)


T7 + New RBS & Terminator + SplintR Ligase Expressing Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 869
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We attached a 6x histidine tag (6x His-Tag) upstream of the SplintR Ligase sequence for purification purposes.


Source

The SplintR Ligase protein amino acid sequence was obtained from the Paramecium bursaria Chlorella virus 1.We optimized the DNA sequence for expression in E. coli and removed the PstI cutting site.

References