Composite
Part:BBa_K3352008:Design
Designed by: Tsuyoshi Misaki Group: iGEM20_TAS_Taipei (2020-10-18)
T7 + New RBS & Terminator + SplintR Ligase Expressing Construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1029
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 869
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We attached a 6x histidine tag (6x His-Tag) upstream of the SplintR Ligase sequence for purification purposes.
Source
The SplintR Ligase protein amino acid sequence was obtained from the Paramecium bursaria Chlorella virus 1.We optimized the DNA sequence for expression in E. coli and removed the PstI cutting site.